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In addition, we also performed conditional Isl1 ablation in developing GnRH neurons. Here, we analyzed the Isl1 genetic lineage in chemosensory neuronal populations in the nasal area and migratory GnRH-1ns in mice using in situ hybridization, immunolabeling a Tamoxifen inducible Isl1Cre ERT and a constitutive Isl1 Cre knock-in mouse lines. One candidate to identify placodal derived neurons in the developing nasal area is the transcription factor Isl1, which was recently identified in GnRH-3 neurons of the terminal nerve in fish, as well as expression in neurons of the nasal migratory mass (MM). Even though the genetic heterogeneity among these cell types is documented, unidentified cell populations arising from the OP remain.
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